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Jun 06, 2020 · sample to be tested. Then, 10g sample to be tested was taken, and put to a 250ml conical flask. in Another conical flask was taken, added with 50ml absolute ethanol and 1ml phenolphthalein indicator, shake well, place into 100 and heatd ed until the ethanol was slightly boiling. The conical
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solvent consisting of methanol-acetonitrile (50:50, v/v%), and 0.1ml internal standard solution of meletin at a concentration of 30 µg/ml. The mixture was vortexed for 4 min and centrifuged at 0 for 4 min. The supernate was transferred to another PE conical tube as plasma sample. The animals were decapitated immediately after blood was
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