Dec 08, 2010 · Thus, the expression of a dominant negative for the transcription factor Creb or a transgene that inhibits CaMKII expression in the γ MB neurons blocks the formation of the γ MB neuron memory trace in parallel with long-term behavioral memory as measured 24 h later.
Feb 23, 2011 · The needles of the syringes were inserted into the air stream. Odors were diluted in paraffin oil to a 0.1 volume concentration. Filter papers (2 cm 2) were soaked with 10 μl of odor solution and placed in the syringes. During the 3 s odor stimulation, only half (2.5 ml) of the syringe air volume was injected into the constant air stream.
Jan 28, 2014 · The homogenate was centrifuged for 5 min at 13′400 rpm. The supernatant was filtered using a syringe filter (millipore, 5-μm pores) into a new Eppendorf cup and then centrifuged again for 5 min at 13′400 rpm. 100 μl of the supernatant was loaded on a 96-well plate (Hartenstein, Würzburg, Germany).
Nov 25, 2015 · After centrifugation to remove protein and other insoluble cell debris, pigment solutions were filtered through 0.2 μm polytetrafluoroethylene membrane syringe filters. Pigments were analyzed by reversed-phase HPLC on a 25 cm × 4.6 mm analytical Discovery C18 column (Supelco, Bellefonte, PA) using an Aijiren Model HPLC system equipped
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Jul 27, 2019 · 5 answers. Feb 16, 2016. In the bottle of a M17 medium they recommend to autoclave the medium without lactose, wait cool down to 50 °C and add (filter sterilized) lactose (or glucose). The medium
The present study was aimed to investigate the composition and contents and the major lipophilic compounds, including the sterols, fatty acids, and tocols of shellfish species. Moreover, to explore the antitumor activity of these lipophilic constituents, their cytotoxicity potentials were determined against five different human cancer cells, including colon carcinoma (HCT116), epithelial
Apr 13, 2011 · A prior screen identified dozens of Drosophila melanogaster mutants that possess defective long-term memory (LTM). Using spaced olfactory conditioning, we trained 26 of these mutant lines to associate an odor cue with electric shock and then examined the memory of this conditioning 24 h later. All of the mutants tested revealed a deficit in LTM compared to the robust LTM observed in control
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Functional optical imaging showed that odor or electric shock stimuli presented to the fly causes transient calcium influx into the two major axon branches of α/β mushroom body (MB) neurons. One pairing of odor and electric shock stimuli
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Jun 26, 2014 · Before 0.2 and 0.22 micron filters became standard, it was thought that filters with an absolute rating of 0.45 micron were thought to be sufficient to strain out even the smallest bacteria. However, the discovery of Brevundimonas diminuta, showed that there were still bacteria capable of passing through a 0.45 micron filter in large quantities.
