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250ul insert yellow

Team:Lambert GA/Experiments – 2018.igem.org Miniprep (using Omega Protocol) 1.1 Grow 1-5mL culture overnight in a 10mL-20mL culture tube. 1.2 Centrifuge at 2500xg for 5 minutes at room temper...
Products Name : Micro Insert Vials for Sale
Dimension : 5x29mm or 6x31mm
Insert Volume : 0.2ml, 0.3ml, 0.4ml
Place of Origin : China
Material : Borosilicate Glass
Package : 100pcs/Pack
Certified : ISO9001:2008
Sample : Provided as Free
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Description

Team:Lambert GA/Experiments - 2018.igem.org

Miniprep (using Omega Protocol) 1.1 Grow 1-5mL culture overnight in a 10mL-20mL culture tube. 1.2 Centrifuge at 2500xg for 5 minutes at room temperature.

Glasgow/Wetlab/Protocols - 2007.igem.org

Combine 50ng of vector with a 3-fold molar excess of insert. Adjust volume to 10ul with dH2O. Add 10ul of 2x Quick Ligation Reaction buffer and mix. Add 1ul of T4 DNA Quick Ligase and mix thoroughly. Centrifuge briefly and incubate at room temperature (25°C) for 5mins. Chill on ice, then transform or store at -20°C.

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