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Reversed-phase HPLC was performed on an RP-18 Merck LiChroSphere 100 column (5 μm, 250 × 4·6 mm i.d.) using a Kontron HPLC apparatus equipped with a series 522 chromatographic pump. Elution of the compounds was monitored at 210 nm using a Kontron DAD 540 diode array detector (DAD) or an Alltech ELSD 500 evaporative light-scattering detector (ELSD) connected to a KromaSystem 2000 integrator
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