Step 4: Inoculate. Remove the piece of mycelium from the agar dish and throw it into the jar. Try to keep the piece of mycelium upstream of all other materials throughout the process. You should try to have the lid of the jar open for as little time as possible.
PES syringe filter membrane has the characteristics of high mechanical strength, it can handle high temperature liquid, and has a large flow rate (better than PTFE), and low protein binding force. With these functions, PES syringe filters can provide excellent performance for biological samples and culture media. The usable pH range is about 3-14.
Reusable Tattler canning lids are the best option for DIY liquid culture and grain spawn lids. Drill two holes in lid, insert injection port in one and syringe filter in the other. Rubber gasket forms an airtight seal. Purchase with stainless steel canning bands, or use your own. Free Shipping when you spend over $150! Size.
Dec 5, 2011 · So this is my take on the most contaminate free lids you can make for quart jars for using a syringe to inoculate. Basically this is just using RTV like other methods use, but you don't need to cut filter discs or get too messy with the RTV. No need to worry about the seal around cut-outs of filter discs, too. 1. Quote:
Jun 17, 2012 · for each jar, remove the jar lid ring and set it aside. flame sterilize the syringe needle, and then wipe the needle with alcohol to remove the flame residue. slide the synthetic filter disc to one side, exposing 1 of the 1/4" holes in the jar lid. inoculate the grain jar through this 1 hole, slide the synthetic filter disc back into place, and
Our synthetic filter discs provide for contaminant-free gas exchange on spawn jar lids. All of our synthetic filter discs have a pore size of 0.3 microns. We carry multiple sizes of filter discs: 29mm (for custom-made spawn jar lids), 70mm (regular mouth jars), 90mm (widemouth jars), 110mm, and 125mm.
Ball Leakproof Storage Lids. These Ball leakproof storage lids are available in both wide and regular mouth sizes and are designed to fit wide mouth and regular mouth canning jars. No more rusty lids! Height Extender Pack for Complete Mushroom Grow Tray $ 59.95 – $ 63.95. All American Pressure Cooker Replacement Parts $ 1.95 – $ 22.95.
This spawn jar lid will fit all regular mouth (70 mm) canning jars. It is intended to be used atop jars of live spawn or mycelium. The lid is equipped with a super heavy duty injection port and a 0.3 µm synthetic filter disc (29 mm diameter) that allows for contaminant-free gas exchange. The lid and all components are 100% autoclavable.
Ask a question about this product. Description. Details. Reviews. Autoclavable plastic jar lid for wide-mouth (90mm) canning jars. These lids will fit all standard widemouth mason jars. The underside of the lid states "Not For Processing" but the lids are able to withstand the temperatures of a pressure cooker or autoclave without issue.
Nov 23, 2020 · You can stick these synthetic filter paper to cover the hole under the wide mouth canning jar lids, and the synthetic filter paper is characteristic with high filter rate, nice for mushroom cultivation. Specifications: Size: 20 mm diameter Color: white Note: Please allow error due to manual measurement. Product information Warranty & Support
High-Quality Scientific Syringe Filter for Mushroom Spawn Jar Lids. These syringe filters provide an ideal amount of fresh air exchange while at the same time preventing airborne particles from contaminating your spawn. Color may vary. Usage Instructions: Simply drill a 5/32 hole in a canning lid and insert the syringe filter with the narrow end snugly into the hole.
Dec 11, 2022 · Lid Material:food grade Polypropylene Lid diameter:86mm Fit for all Wide Mouth Mason Jar The second generation Lid comes with two self healing injection for injecting culture to inoculate the substrate more average and a PTFE syringe filter for avoid contamination without glue,more healthy for mushroom growing.
May 20, 2019 · Liquid Culture Jar Lids 6 Pack - Autoclavable Plastic Wide Mouth Jar Lids with Heavy Duty Syringe Injection Port for Mushroom Cultivation Visit the Liquid Fungi Store 20 ratings $3299 ($5.50 / Item) About this item autoclavable polypropylene plastic lid super heavy duty medical grade syringe injection port 0.45um air exchange filter
Synthetic Filter Discs. $ 0.85 – $ 113.95. These synthetic filter discs fit precisely on a variety of canning jars that are commonly used by today’s mushroom cultivators. Usage of these discs allows a fresh, constant air exchange but prevents the entrance of unwanted contaminates. Size.
Clear. Single syringe filter. $ 1.89. Add to cart. Categories: Growing Supplies, Lab & Culture Work, Liquid Culture Supplies SKU: LAB-SF-1. Additional information. Syringe filter (13 mm diameter, 0.22 µm). Installed into a canning jar lid, the filter will keep contaminants out, while allowing for adequate gas exchange your liquid cultures.
