Kerr wide mouth 1/2 pint canning jars. Ball regular mouth 1/2 pint canning jars. Step 1: Prepare the tops of the culture jars so that they can be in place on the jars when inoculating the jars with the spore syringe. Part of the reason this system works so well in the non-sterile kitchen environment is the fact that the sterilized substrate is
Dec 04, 2009 · Now shake the syringe really well, using the lighter heat the needle of the syringe till its red hot now proceed to inoculate the jars using a 1/4 cc of solution per hole inoculating towards the glass. That’s 1 cc per jar. After every jar heat the needle back till its red hot and repeat. Incubation.
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Drill two holes in the lid, then insert an injection port into one hole and a syringe filter into the other to make your own reusable airport lid. Fill canning jar with cooked rye grain, or other substrate, sterilize in pressure canner, then injection with your favourite edible or medicinal mushroom liquid culture.
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Feb 13, 2014 · The syringe filters don't work for the same reason a small piece of synthetic filter disc won't work. If you get a sheet of thick felt and cut it in small squares, it will filter the air fine and provide all the gas exchange you need.
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May 27, 2011 · Shake your spore syringe to distribute the spore solution inside and then flame sterilize the needle. 3. Place the needle into the hole in your substrate jar at an angle so the tip of the needle is up against the glass. Then GENTLY inject 1/4th of a CC into the jar. Remove the needle and repeat for the other 3 holes.
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Jan 06, 2011 · Usually this is accomplished when you open it to add the coffee grounds for the day. You can also give the mushrooms air flow by using a canning jar and replacing the sealing portion of the lid with a coffee filter (unused) or a piece of fabric. Just keep the jar in a cool, dry location while it is growing.
A 1/2 pint canning jar similar to that used for the cultures is fine. Sterilize both the jar and the lid. Place the harvested cap in the sterilized, wide mouth jar. Seal the top with the lid and wait until a spore print has been generated on the bottom of the glass. Open the jar and remove the mushroom cap. Add 3/4 cup of sterilized water.
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