Determination of Gestodene in Human Plasma by SLE-LC-MS/MS
Plasma by SLE-LC-MS/MS Using a Solid Core HPLC Column Krishna Rao Dara and Tushar N. Mehta Centre of Excellence for Asia Pacific Laboratory, Thermo Fisher Scientific, Ahmedabad, India Introduction Gestodene is a synthetic form of progesterone that is used in oral contraceptives in combination with a synthetic estrogen.
Product information: T4 DNA Ligase, #EL0011
DNA INSERT LIGATION INTO VECTOR DNA Sticky-end ligation 1. Prepare the following reaction mixture: Linear vector DNA 20 -100 ng Insert DNA 1:1 to 5:1 molar ratio over vector 10X T4 DNA Ligase Buffer 2 µL T4 DNA Ligase Total v1 Weiss U Water, nuclease -free (#R0581) to 20 µL Total volume 20 µL 2. Incubate 10 min at 22°C. 3.
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trols was measured by reverse phase HPLC (RP-HPLC). Samples were analyzed using an Aijiren HPLC System with a quaternary pump system. HPLC columns used were Transgenomic OligoSep HC (5.5–6.0 micron, porous, alkylated polymer (DVB), 4.6 x 50 mm, P/N NUC-99-3560)). Mobile phases used were 0.1 M TEAA (triethylamine acetate)
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Amino Acid Analysis of Mammalian Cell Culture Medium by
Thermo Fisher Scientific, Germering, Germany Keywords AccQ –Tag™, protein hydrolysate analysis, ACQ, pre-column derivatization, bioprocessor, OPA-FMOC Amino acid analysis of mammalian cell culture medium by liquid chromatography with UV and fluorescence detection and derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate
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The world leader in serving science HPLC Tips and Tricks Tiziana Ladisa Sales Support Specialist for Chromatography Italy Thermo Fisher Scientific, Rodano (MI)
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